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LEGO Batman (Bootleg, Sega Mega Drive)
In various levels, you can find a couple of small cameo appearances of our little friend Luigi (though the tile art is slightly inaccurate).
...what do you mean that's Mario? How would you know, wise guy?
(glad to hear you all like this anyways here's the individual assets. if you need to use these for some reason go ahead but just credit me @DavidPCM_)
Unused Fighter - Super Smash Bros. Brawl
Within the game's files, there's a file indicating an unused fighter dubbed as "Retro Luigi", being a unique retro rep animated purely by sprites.
He was going to replace Mr. G&W initially, but was scrapped after much reconsideration.
Intro Cutscene - Hotel Mario
This was going to be an easter egg within the game that happens if your CD-i's date was set to Sep. 13 1985. It was cut from the final for initially scaring kids and it also wasn't a valid date to be set on a stock CD-i.
(Collab with @OctoHorizon!)