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One of the frames of Wario's animation while returning from a level to the main menu in Wario Land: Shake It.
During Luigi's somersault animation in Mario & Luigi: Dream Team, one frame consists of a superposition of two Luigi sprites.
Whenever Bowser punches the ground in Super Mario Galaxy 2, the arm he punches with expands to roughly twice its usual size.
In Super Mario World, Mario has special tilted sprites that are visible for one frame when he is running up a Triangle Block.
Removing the mustache from Toadsworth's model in Super Mario Sunshine reveals that he has no mouth.
Repeatedly refusing to buy information from the info vendor in Mario & Luigi: Superstar Saga will cause the game to glitch and freeze.
Sprite of Zero Suit Samus from the Metroid microgame in WarioWare: D.I.Y, drawn by Yoshio Sakamoto, co-creator of the Metroid series.
All the friendship backgrounds from the Bouncy Ball souvenir in WarioWare: Twisted.
In Super Mario Bros. Deluxe, selecting any print menu with Peach present results in a partial afterimage of Peach appearing for one frame.
At 975x1273 pixels, this Big Bob-omb sprite from Paper Mario: Color Splash is the biggest individual sprite from any Paper Mario game.