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White Pearl from Power Stone 2 (2000), has little to no Rule34.
Captain VaVa LaVoom from (partially lost) Elmo Aardvark: Outer Space Detective, has little to no Rule34.
Ruby Heart from Marvel vs. Capcom 2: New Age of Heroes (2000), has little to no Rule34; on a side note, she is neither from Marvel nor Capcom, but an original character for the game.
Cate Archer from The Operative: No One Lives Forever (2000), has little to no Rule34.
Neptuna from Plants vs. Zombies Heroes (2016), has little to no Rule34.