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For unknown reasons, when Fangs fly, they leave a short dashed line behind them.
In Yoshi Touch & Go, Watermelons, when eaten, give Yoshi five eggs.
The clocks in this room depict four o'clock (04:00 or 16:00).
Waluigi speaks in clear English, but with slang. Waluigi drops the "g"'s in his sentences, or makes use of the word "ain't" on a frequent basis. Waluigi also has a habit of name-calling, as well as saying "Hey, dummy!" to his own teammates in Mario Strikers Charged.
This is the first Mario game to have extensive voice acting and full dialogue.
As Wario comments in the game's North American instruction booklet, these enemies smell like feet.
Yoob bears a loose resemblance to Barney the Dinosaur, the host of Barney & Friends, an educational children's program.