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2014 - Jojo's Bizarre Adventure: Stardust Crusaders
2016 - Ace Attorney, Jojo's Bizarre Adventure: Diamond is Unbreakable
(two entries to make up for the lack of one for 2017)
Wait, I just realized that I think he counts, too
Another Honorable "I Don't Know if They Count As Villains" Mentions round!
The Brain, Animaniacs (1993-1998, 2020- ) and Pinky & the Brain (1995-1998)
The Witch, Into the Woods (1987)
Jasper, Steven Universe (2013-2019)
The Medic, Team Fortress 2 (2007)
Two entries in one tweet!
Archie and Maxie, Pokemon Omega Ruby/Alpha Sapphire (2014)
I think these remakes made both characters even more entertaining, and their designs here are so great I was kinda mad they weren't used when they showed up in Ultra Sun/Ultra Moon
Risotto Nero, Jojo's Bizarre Adventure: Golden Wind (manga 1995-1999, anime 2018-2019)
I thought he was a better, more interesting antagonist than Diavolo.